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pi3k-(p110alpha[h1047r]/p85alpha),-20ug

PI3K (p110alpha[H1047R]/p85alpha), 20ug

by Promega
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    Recombinant Human Phosphoinositide 3-Kinases (PI3Ks)

    Phosphatidylinositol (PI) and its phosphorylated derivates, collectively called phosphoinositides, are important second messengers that are critical as signaling molecules and for cellular membrane remodeling. These derivatives are generated by a family of kinases called phosphoinositide lipid kinases (PIKs). 

    We offer lipid kinase enzymes, substrates and detection systems for performing phosphoinositide lipid kinase (PIK) reactions using a luminescent ADP-detection platform, the ADP-Glo™ Kinase Assay. The purified human recombinant PI3K enzymes are components of the PI3K Class I Profiling Kit (Cat.# V1690) that are available separately. 

    Sources

    PI3K (p110?/p85?), 20ug: Complex of recombinant N-terminal FLAG-tagged full-length human p110? (GenBank® Accession Number U79143) and human p85? (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

    PI3K (p110?[E545K]/p85?), 20ug:     Complex of recombinant N-terminal FLAG-tagged full-length human p110? (GenBank® Accession Number U79143) and human p85? (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

    PI3K (p110?[H1047R]/p85?), 20ug:     Complex of recombinant N-terminal FLAG-tagged full-length human p110? (GenBank® Accession Number U79143) and human p85? (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

    PI3K (p110?/p85?), 20ug: Complex of recombinant N-terminal FLAG-tagged full-length human p110? (GenBank® Accession Number NM_006219) and human p85? (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

    PI3K (p120?), 20ug:     Recombinant N-terminal His-tagged full-length human p120? (GenBank® Accession Number NM _02649) expressed in Sf9 insect cells.

    PI3K (p110?/p85?), 20ug:     Complex of recombinant N-terminal GST-tagged full-length human p110? (GenBank® Accession Number NM_005026) and human p85? (no tag; GenBank® Accession Number XM_043865). Co-expressed in Sf9 insect cells.

  • Protocols

    Complete Protocol

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    ADP-Glo™ Lipid Kinase Assay

    PDF (747 KB)

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    PI3K (p110?/p85?) Protocol

    PDF (511 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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