ADP-Glo Kinase Assay + IGF1R Kinase Enzyme System

by Promega
Available SKUs: V9401 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Kinase-background-v2light

    Convenient, Scalable Kinase Profiling

    The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The IGF1R Kinase Enzyme System contains:

    • IGF1R Kinase, 10ug (Human, recombinant; amino acids 960–end). MW: ~53kDa.
    • IGF1Rtide (KKKSPGEYVNIEFG) Substrate; derived from human IRS-1 protein residues 891–902.
    • Reaction Buffer, DTT, MnCl2.

    Recombinant human IGF1R (amino acids 960–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. IGF1R (Insulin-like Growth Factor 1) is a transmembrane tyrosine kinase receptor that is activated by IGF-1 and by the related growth factor IGF-2. IGF1R mediates the effects of IGF-1 and plays an important role in growth and anabolic effects in adults. The IGF1R is implicated in several cancers, most notably breast cancer, where it is highly overexpressed and functions as an anti-apoptotic agent by enhancing cell survival. In many instances, the anti-apoptotic properties of IGF1R overexpression allow cancerous cells to resist the cytotoxic properties of chemotherapeutic drugs or radiotherapy.

    IGF1R NCBI Database Entry.

    The IGF1R Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

    Use with ADP-Glo™ Kinase Assay

    The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

     

    See all Kinase Enzyme Systems available from Promega.

  • Protocols

    Complete Protocol

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    IGF1R Kinase Enzyme System Product Information

    PDF (1 MB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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