pmirGLO Dual-Luciferase miRNA Target Expression Vector
The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization using the Dual-Luciferase® Assay and selection for stable cell lines.
- miRNA detection.
- miRNA binding site analysis.
- Transcript stability study.
- 3´-UTR analysis.
pmirGLO Dual-Luciferase miRNA Target Expression Vector Protocol
PDF (294 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.