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pmirGLO Dual-Luciferase miRNA Target Expression Vector

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The pmirGLO Vector is designed to quantitatively evaluate microRNA (miRNA) activity by the insertion of miRNA target sites downstream or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary reporter gene; reduced firefly luciferase expression indicates the binding of endogenous or introduced miRNAs to the cloned miRNA target sequence. This vector is based on Promega dual-luciferase technology, with luc2 used as the primary reporter to monitor mRNA regulation and Renilla luciferase (hRluc-neo) acting as a control reporter for normalization using the Dual-Luciferase® Assay and selection for stable cell lines.

    Applications

    • miRNA detection.
    • miRNA binding site analysis.
    • Transcript stability study.
    • 3´-UTR analysis.
    Normalized luciferase activity using the pmirGLO Vector with an mi-R21 target sequence.
    Normalized luciferase activity using the pmirGLO Vector with an mi-R21 target sequence.

    Sequence Information

    pmirGLO Vector GenBank® Accession Number FJ376737 and vector sequence text file.

    Mechanism of action of the pmirGLO Vector.
    Mechanism of action of the pmirGLO Vector.
  • Protocols

    Complete Protocol

    Download PDF

    pmirGLO Dual-Luciferase miRNA Target Expression Vector Protocol

    PDF (294 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations