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PNGase F

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • PNGase F, Recombinant Glycosidase

    PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli. PNGase F has a molecular weight of 36kDa. PNGase F catalyzes the cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins (see figure). PNGase F will not remove oligosaccharides containing Alpha-(1,3)-linked core fucose commonly found on plant glycoproteins.

    Unit Definition: One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute at 37°C. One Promega unit is equal to 1 IUB milliunit.

    Molecular Weight: PNGase F has a molecular weight of approximately 36kDa.

    Physical Form: PNGase F is supplied as a liquid in 20mM Tris-HCl (pH 7.5 at 25°C), 50mM NaCl and 5mM EDTA at a concentration of 10,000u/ml.

    Applications
    • Characterizing whether the protein is glycosylated
    • Determining the location of glycosylation on the protein
    • Characterizing the glycan structure
    • Protein trafficking
    Cleavage specificity of PNGase F on N-Glycans.
    Cleavage specificity of PNGase F on N-Glycans.
  • Protocols

    Complete Protocol

    Download PDF

    PNGase F Protocol

    PDF (115 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +2C TO +10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.