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PolyATtract System 1000 without Magnetic Stand

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The PolyATtract® System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolations. This system uses the MagneSphere® technology for the purification of poly(A)+ RNA, eliminating the need for oligo(dT) cellulose columns. The increased yield of mRNA using this system allows the detection of low-copy-number mRNAs in relatively small amounts of material using Northern blot analysis. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation, cDNA synthesis, PCR analysis, ribonuclease (RNase) protection assays, primer extension and Northern blots.

    The MagneSphere® Technology Magnetic Separation Stands can be used in conjunction with any of the PolyATtract® Systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules.

  • Protocols

    Complete Protocol

    Download PDF

    PolyATtract® System 1000 Technical Manual

    PDF (1 MB)

    Quick Protocols

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    PolyATract System 1000 Quick Protocol FB047

    PDF (138 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +2C TO +10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources