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STR Analysis Using the PowerPlex® 16 HS System
The PowerPlex® 16 HS System is a multiplex STR system for use in DNA typing. This system co-amplifies the loci D18S51, D21S11, TH01, D3S1358, Penta E (labeled with fluorescein); FGA, TPOX, D8S1179, vWA and Amelogenin (labeled with TMR); CSF1PO, D16S539, D7S820, D13S317, D5S818 and Penta D (labeled with JOE). This multiplex includes all 13 CODIS STR markers, Amelogenin for gender determination and two low-stutter, highly discriminating pentanucleotide STR markers. The loci included in the PowerPlex® 16 HS System are more discriminating than competitive systems and are ideal for resolving partial matches or challenging familial cases.
The PowerPlex® 16 HS System is easy to use and includes size standard, amplification-grade water and Taq DNA polymerase already in the master mix. All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection. This system produces full profiles with 100pg of DNA, giving you confidence in the analysis of precious samples. The PowerPlex® 16 HS System is more tolerant of PCR inhibitors than competing STR systems and the previous version of the PowerPlex® 16 System, increasing your success rate and reducing the need for re-amplification. The PowerPlex® 16 HS System master mix is more tolerant of PCR inhibitors than competitive STR systems.
Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® ID and ID-X software and are available for download. Primer sequences, dyes and ladders are all unchanged from the PowerPlex® 16 System, providing concordance with existing databases. The PowerPlex® 16 HS System is compatible with ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Konjhodic, R. et al. (ICMP) (2004) Comparative study of Penta D and Penta E with D2 and D19, IDENTITY Newsletter5,1–2.