Skip to content

Protease Inhibitor Cocktail, 50X

by Promega
Login to view price.
Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Best-in-Class Protease Inhibition

    Prevent protein degradation after cell lysis. This mixture of six different inhibitors covers a wide range of target proteases. The inhibitor cocktail is EDTA-free and provided as a powder, ready for reconstitution in 1ml of either 100% ethanol or DMSO to obtain a 50X working solution.

    Protease Inhibitor Cocktail provides the best-in-class level of protease inhibition. It works with a wide array of protein fusion tags (e.g., Flag®, His tag, GST tag) and capture technologies and is ideally suited for HaloTag® Technology-based approaches.

    Applications

    • Protein purification from mammalian cell culture.
    • Protein purification from insect cell culture.
    • Analysis and capture of protein:protein, protein:DNA interactions.

    References

    1. Ohana, R.F. et al. (2011) HaloTag-based purification of functional human kinases from mammalian cells. Protein Expr. Purif. 76, 154–64.
    2. Lee, J.-S. et al. (2011) Molecular and functional characterization of aryl hydrocarbon receptor repressor from the chicken (Gallus gallus): Interspecies similarities and differences. Toxicol. Sci. 119, 319–34.
    3. Nakashima, R. et al. (2010) The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody. Rheumatology 49, 433–40.
    Representative inhibition of serine protease with protease inhibitor cocktail at manufacturer's-specified active concentration (1X dilution).
    Representative inhibition of serine protease with protease inhibitor cocktail at manufacturer's-specified active concentration (1X dilution).
  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles

    Citations