Skip to content

PureYield RNA Midiprep System

by Promega
Login to view price.
Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Pure Total RNA. No Organic Solvents or Ethanol Precipitation

    The PureYield™ RNA Midiprep System allows rapid purification of total RNA with undetectable levels of genomic DNA contamination without using DNase. A novel combination of reagents, membranes and protocol leads to yields of up to 1mg of total RNA without organic solvents, protease digestions or alcohol precipitations. One kit can be used to isolate pure total RNA from a wide variety of sample types, such as tissues, cultured cells, bacteria, yeast, plants and blood. The protocol also can be adapted for other sample types.

    Other commonly used RNA isolation methods provide total RNA that is contaminated with genomic DNA  that interferes with sensitive methods, such as real-time RT-PCR and microarray analysis. The PureYield™ RNA Midiprep System avoids this problem by selectively removing genomic DNA prior to total RNA purification. The eluted total RNA is free of detectable DNA and ready for use in sensitive downstream applications.

    The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold) formats.

    • Purified total RNA with undetectable genomic DNA contamination improves results in downstream applications.
    • Purifying total RNA without the use of DNase treatment reduces steps during purification and in downstream applications.

    Process Multiple Sample Types

    Blood
    Sample Type Promega External Comments
    Blood and blood cells A general protocol for total RNA isolation from blood cells is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279. Additional resources are listed below.
    Human blood Yes A protocol is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource: Brisco, P. et al. (2006) Promega Notes 92, 14–9
    Mammalian Tissues
    Sample Type Promega External Comments
    Mammalian tissues A general protocol for total RNA isolation from tissue is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resources are listed below.
    Rat liver Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Rat lung Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Rat kidney Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Rat spleen Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Rat brain Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Rat heart Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Rat muscle Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Bovine liver Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Bovine spleen Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Bovine heart Yes Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Plant Tissues
    Sample Type Promega External Comments
    Plant tissues Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Canola leaf Yes Follow the standard protocol for plant tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Corn/maize leaf, stem and silk tissue Yes Yes Follow the standard protocol for plant tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Cultured Cells
    Sample Type Promega External Comments
    Cultured cells Yes A general protocol for total RNA isolation from mammalian cultured cells is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Bacteria
    Sample Type Promega External Comments
    Bacteria A general protocol for total RNA isolation from Gram-positive and Gram-negative bacteria is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resources are listed below.
    Escherichia coli Yes Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Bacillus subtilis Yes Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Bacillus sphaericus Yes Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Bacillus globigii Yes Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Artherobacter luteus Yes Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Micrococcus luteus Yes Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Staphylococcus aureus Yes Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Other Organisms and Tissues
    Sample Type Promega External Comments
    Saccharomyces cerevisiae Yes A protocol is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Brisco, P. et al. (2006) Promega Notes 92, 14–9.
    Chicken liver Yes Follow the standard protocol in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Miscellaneous
    Sample Type Promega External Comments
    phenol:chloroform-extracted RNA samples Yes A protocol for clean up for phenol:chloroform-extracted RNA samples is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Additional resource:
    Mandrekar, M. et al. (2006) Promega Notes 94, 7–11.
    RNA pellet from an alcohol precipitation Yes A protocol for clean up of RNA pellets is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
    Purified RNA in an aqueous solution Yes A protocol for cleaning up RNA in an aqueous solution is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
  • Protocols

    Complete Protocol

    Download PDF

    PureYield™ RNA Midiprep System Technical Manual

    PDF (1 MB)

    Quick Protocols

    Download PDF

    PureYield RNA Midiprep System Quick Protocol

    PDF (66 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles

    Citations