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pvui

PvuI

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources

  • Cut Site:

    CG   AT?CG
    GC?TA   GC

    Usage Notes:

    1. Religation of DNA fragments with dam methylation is difficult.
    2. Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

    Incubation Conditions: Buffer D. 37°C.

    Source: Proteus vulgaris.

    Storage Buffer: 10mM Tris-HCl (pH 7.3 @ 25°C), 50mM NaCl, 0.1% Triton® X-100, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    10–25% 25–50% 50–75% 100% <10%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    3 7 0 2 1 1

  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other