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Reporter Lysis 5X Buffer

by Promega
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  • Specifications
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  • Reporter Lysis 5X Buffer is used with the Luciferase Assay System with Reporter Lysis Buffer (Cat.# E4030), developed for reporter quantitation in mammalian cells. Reporter Lysis Buffer (RLB) is a mild lysis agent and requires a single freeze-thaw cycle to achieve complete cell lysis. RLB is compatible with two reporter enzyme assays: ?-galactosidase (?-gal) and luciferase. The Reporter Lysis Buffer allows ?-gal and luciferase assays to be performed on cell lysates of cells cotransfected with vectors carrying these reporter genes. In cell lines tested at Promega, ?-gal activity in cells lysed with Reporter Lysis Buffer is significantly higher than activity in lysates prepared by the freeze-thaw method.


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    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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