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Riboprobe Combination System-T3/T7 RNA Polymerase

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The Riboprobe® Combination Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe® Combination Systems include the RNA polymerases, all of the required reagents (excluding radioisotope) for performing transcription reactions in vitro and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template following transcription.

    Applications of the Riboprobe® Systems include:

    • Generation of high-specific-activity RNA probes.
    • Generation of microgram quantities of RNA transcripts.
    • Generation of RNA processing substrates.
    • Generation of antisense RNA.
  • Protocols

    Complete Protocol

    Download PDF

    Riboprobe® In Vitro Transcription Systems Technical Manual

    PDF (1 MB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations