RsaI

Cut Site:

GT^?AC
CA_?TG

Note: For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.

Incubation Conditions: Buffer C. 37°C.

Source: Rhodopseudomonas sphaeroides.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: