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S1 Nuclease

by Promega
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  • S1 Nuclease is an endonuclease that degrades ssDNA and RNA. The enzyme is used to remove protruding single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts. S1 Nuclease is provided with 10X Reaction Buffer: 0.5M sodium acetate (pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO4.

    References

    1. Vogt, V.M. (1973) Eur. J. Biochem. 33, 192–200.
    2. Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 760–4.
    3. Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75, 1274–8.

    S1 Nuclease

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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