ADP-Glo Kinase Assay + p38 delta Kinase Enzyme System
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The p38? Kinase Enzyme System contains:
- p38? Kinase, 10ug (Human, recombinant full-length). MW: ~71kDa.
- p38 Substrate (IPTTPITTTYFFFKKK).
- Reaction Buffer, DTT.
Recombinant full-length human p38? was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38? (SAPK4) is a member of the p38 MAPK family and is activated by chemical and environmental stresses as well as by proinflammatory cytokines. p38? has a TGY dual phosphorylation motif and is activated in response to cellular stresses and proinflammatory cytokines. MAP kinase kinases 3 and 6 can phosphorylate and activate this kinase. Transcription factor ATF2 and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.
The p38? Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.
p38 delta Kinase Enzyme Protocol
PDF (908 KB)
Certificate of AnalysisLookup Certificate of Analysis
LESS THAN -65C
For Research Use Only. Not for Use in Diagnostic Procedures.