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SacII

by Promega
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Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    CC   GC?GG
    GG?CG   CC

    Incubation Conditions: Buffer C. 37°C.

    Source: Streptomyces achromogenes.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    100%* 50–75% 100% 50–75% <10%

    *Buffer A is not recommended for use with supercoiled plasmid due to incomplete cutting.

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    4 33 1 0 0 0
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other