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SalI

by Promega
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Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    G?TCGA   C
    C   AGCT?G

    Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

    Usage Notes:

    1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.
    2. Ends generated by SalI can be directly ligated to ends generated by XhoI. Neither recognition site is regenerated in the ligation product.

    Incubation Conditions: Buffer D. 37°C.

    Source: Streptomyces albus G.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    <10% 10–25% 25–50% 100% <10%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    2 3 0 1 1 1
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other