Sequencing Grade Modified Trypsin

by Promega
Available SKUs: V5111 | V5117 |
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Description

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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fragments detected by mass spectrometry. Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.

    The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilized. It is resistant to mild denaturing conditions such as 0.1% SDS, 1M urea or 10% acetonitrile and retains 50% of its activity in 2M guanidine HCl. The activity of trypsin is decreased when acidic residues are present on either side of a susceptible bond. If proline is at the carboxylic side of lysine or arginine, the bond is almost completely resistant to cleavage.

    Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

    Recommended Reaction Buffer: 50mM NH4HCO3 (pH 7.8).

    References
    1. Keil-Dlouha, V.V. et al. (1971) FEBS Letters 16, 291–5.
    2. Rice, R.H. et al. (1977) Biochem. Biophys. Acta 492, 316–21.
    Cut sites for Arg-C, Sequencing Grade; Glu-C, Sequencing Grade; Asp-N, Sequencing Grade; Trypsin Gold, Mass Spectrometry Grade; Sequencing Grade Modified Trypsin; Immobilized Trypsin; Chymotrypsin, Sequencing Grade; Endoproteinase Lys-C, Sequencing Grade; rLys-C, Mass Spec Grade.
    Cut sites for Arg-C, Sequencing Grade; Glu-C, Sequencing Grade; Asp-N, Sequencing Grade; Trypsin Gold, Mass Spectrometry Grade; Sequencing Grade Modified Trypsin; Immobilized Trypsin; Chymotrypsin, Sequencing Grade; Endoproteinase Lys-C, Sequencing Grade; rLys-C, Mass Spec Grade.
  • Protocols

    Complete Protocol

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    Sequencing Grade Modified Trypsin Product Information

    PDF (120 KB)

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    Sequencing Grade Modified Trypsin, Frozen, Product Information

    PDF (80 KB)

  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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