Skip to content

SgfI

by Promega
Login to view price.
Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    GCG  AT?CGC
    CGC?TA  GCG

    Notes:

    1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.
    2. Sensitive to CpG methylation.
    3. SgfI is inactivated by freezing at –70°C.
    4. Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

    Incubation Conditions: Buffer C. 37°C.

    Source: Streptomyces fulvissimis.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1% Thesit®, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    25–50% 25–50% 100% <10% <10%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    0 1 0 0 0 0
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other