- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.
- Sensitive to CpG methylation.
- SgfI is inactivated by freezing at –70°C.
- Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.
Incubation Conditions: Buffer C. 37°C.
Source: Streptomyces fulvissimis.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1% Thesit®, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™ 25–50% 25–50% 100% <10% <10%
Frequency of Cutting:
? Ad-2 ?X174 pUC18 M13mp18 pBR322 0 1 0 0 0 0
Restriction Enzyme Protocol
PDF (410 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.