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SphI

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    G  CATG?C
    C?GTAC  G

    Incubation Conditions: Buffer K. 37°C.

    Source: Streptomyces phaeochromogenes.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 100mM sodium citrate, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D K MULTI-CORE™
    75–100% 75–100% 100%* 75–100% 100% 10–25%

    *Buffer C is not recommended due to potential star activity.

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    6 8 0 1 1 1
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other