ADP-Glo Kinase Assay + PAK3 Kinase Enzyme System

by Promega
Available SKUs: V4081 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Kinase-background-v2light

    Convenient, Scalable Kinase Profiling

    The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PAK3 Kinase Enzyme System contains:

    • PAK3 Kinase, 10ug (Mouse, recombinant full-length). MW: ~89kDa.
    • Native Swine Myelin Basic Protein (MBP) Substrate.
    • Reaction Buffer, DTT.

    Recombinant full-length mouse PAK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PAK3 is a member of the family of serine/threonine p21-activating kinases that serve as targets for the small GTP-binding proteins Cdc42 and RAC. The PAK family of proteins has been implicated in a wide range of biological activities and its critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK3 seems to be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in the PAK3 gene has been linked to nonsyndromic X-linked mental retardation.

    PAK3 NCBI Database Entry.

    The PAK3 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

    Use with ADP-Glo™ Kinase Assay

    The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

     

    See all Kinase Enzyme Systems available from Promega.

  • Protocols

    Complete Protocol

    Download PDF

    PAK3 Kinase Enzyme Protocol

    PDF (926 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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