Skip to content

SV Total RNA Isolation System

by Promega
Login to view price.
Size
  • Overview
  • Protocols
  • Specifications
  • Resources
  • Isolate RNA with a Simple, 1-hour Procedure

    The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour. Using this membrane-based purification system, up to 60mg of tissue can be processed per purification, depending on tissue type. The system incorporates a DNase treatment step directly on the minicolumn membrane. This step substantially reduces genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, and there is no DNase carryover in the final RNA preparation

    Sample Types Processed with the SV Total RNA Isolation System

    Blood
    Sample Type Promega External Comments
    Fresh whole blood Yes Yes A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
    Hara, N. et al. (2002) Clin. Cancer Res. 8, 1794–9.
    PubMed ID #12060619.
    Human blood plasma and serum Yes Yes A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resource:
    Fresh or once-frozen human serum (100ul) was added to 175ul of SV RNA Lysis Buffer, then the remainder of the SV Total RNA Isolation System protocol was followed.
    Chen, X.Q. et al. (2000) Clin. Cancer Res. 6, 3823–6.
    PubMed ID #11051224.
    Human leukocytes Yes A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
    Winter flounder red blood cells Yes Pedersen, S.F. et al. (2003) Am. J. Physiol. Cell Physiol. 284, C1561–76.
    PubMed ID #12734109.
    Human peripheral blood mononuclear cells Yes Yawalker, N. et al. (1998) J. Invest. Dermatol. 111, 1053–7.
    PubMed ID #9856816.
    Escoffier, C. et al. (1999) J. Virol. 73, 1695–8.
    PubMed ID #9882382.
    Primary CD4+ human T lymphocytes Yes Secchiero, P. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 14620–5.
    PubMed ID #11114167.
    Viral RNA from human plasma Yes Personal communication. Contact Promega Technical Services for more information.
    Mammalian Tissues
    Sample Type Promega External Comments
    Mammalian tissues A general protocol for total RNA isolation from tissue is provided in the SV Total RNA Isolation System Technical Manual #TM048. Additional resources are listed below
    Mouse or rat brain Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Kephart, D. Measuring gene expression from mammalian brain tissue. eNotes
    Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
    Kephart, D. and Hooper, K. (1998) Promega Notes 69, 17–9.
    Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse thoracic dorsal root ganglion neurons Yes Ekström, P.A.R. et al. (2003) Mol. Cell. Neurosci. 22, 383–95.
    PubMed ID #12691739.
    Mouse central nervous system Yes Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002.
    PubMed ID #11106572.
    Mouse small intestine Yes Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002.
    PubMed ID #11106572.
    Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse muscle Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Sakai, H. et al. (1999) J. Physiol. 519, 323–33.
    PubMed ID #10457052.
    Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse or rat liver Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Sakai, H. et al. (1999) J. Physiol. 519, 323–33.
    PubMed ID #10457052.
    Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse kidney Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Attmane-Elakeb, A. et al. (2000) J. Biol. Chem. 275, 33548–53.
    PubMed ID #10942780.
    Mouse spleen Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002.
    PubMed ID #11106572.
    Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse embryonic heart, brain and skeletal muscle Yes Agarkova, I. et al. (2000) J. Biol. Chem. 275, 10256–64.
    PubMed ID #10744711.
    Mouse or rat lung Yes Yes Agarkova, I. et al. (2000) J. Biol. Chem. 275, 10256–64.
    PubMed ID #10744711.
    Rat pancreas Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
    Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse or rat heart Yes Yes Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
    Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse colon Yes Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse stomach Yes Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse thymus Yes Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
    PubMed ID #10037740.
    Mouse eyes Yes Lucas, R.J. et al. (1999) Science 284, 505–7.
    PubMed ID #10205062.
    Mouse retina Yes Freedman, M.S. et al. (1999) Science 284, 502–4.
    PubMed ID #10205061.
    Rat whole cochlea, modiola and cochlear sensorineural epithelial/lateral walls Yes Stöver, T. et al. (2000) Brain Res. Mol. Brain Res. 76, 25–35.
    PubMed ID #10719212.
    Human muscle Yes Roth, S.M. et al. (2002) Physiol. Genomics 10, 181–90.
    PubMed ID #12209020.
    Human placenta Yes Neuschäfer-Rube, F. et al. (2003) Biochem. J. 371, 443–9.
    PubMed ID #12519077.
    Gonzalez-Perrett, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 1182–7.
    PubMed ID #11252306.
    Human skin Yes Yawalker, N. et al. (1999) J. Invest. Dermatol. 113, 43–8.
    PubMed ID #10417617.
    Yawalker, N. et al. (1998) J. Invest. Dermatol. 111, 1053–7.
    PubMed ID #9856816.
    Human brain Yes Miyasaka, T. et al. (2001) Am. J. Pathol. 158, 373–9.
    PubMed ID #11159174.
    Primary human peritoneal mesothelial cells Yes Cronauer, M.V. et al. (1999) Am. J. Pathol. 155, 1977–84.
    PubMed ID #10595927.
    Human cerebrovascular tissue Yes Cronauer, M.V. et al. (1999) Am. J. Pathol. 155, 1977–84.
    PubMed ID #10595927.
    Human adenoma tissue Yes Jaquet, P. et al. (2000) J. Clin. Endocrinol. Metab. 85, 781–92.
    PubMed ID #10690891.
    Human hair follicle Yes Use fresh samples and at least 10 or more hair roots. Align the hairs, and cut the root end from the hair and quickly process using the SV RNA Isolation System. The amount of RNA is very small and can generally only be detected by RT-PCR. Contact Promega Technical Services for more information.
    Ferret aorta Yes Gangopadhyay, S.S. et al. (2002) Biochem. J. 372, 347–57.
    PubMed ID #12603201.
    Feline oral mucosa Yes Harley, R. et al. (1999) Clin. Diag. Lab. Immunol. 6, 471–8.
    PubMed ID #10391845.
    Plant Tissues
    Sample Type Promega External Comments
    Plant tissues A general protocol for total RNA isolation from plant tissue is provided in the SV Total RNA Isolation System Technical Manual #TM048. Additional resources are listed below.
    Tomato leaf Yes Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Shenoi, H. and Kephart, D. Titration of tomato leaf material used as starting material for SV total RNA isolation. eNotes
    Kobs, G. (1998) Promega Notes 68, 28–31.
    Arabidopsis thaliana leaf Yes Yes Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Technically Speaking: SV Total RNA Isolation System. Promega Notes 68, 16–9. Caldelari, D. et al. (2001) Plant Physiol. 126, 1416–29. PubMed ID #11500541.
    Arabidopsis thaliana flower, stem and root Yes Caldelari, D. et al. (2001) Plant Physiol. 126, 1416–29.
    PubMed ID #11500541.
    Tobacco leaf Yes Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Technically Speaking: SV Total RNA Isolation System. Promega Notes 68, 16–9.
    Sugar beet 14-day seedling, cotyledon, hypocotyl, 46-day plant and 90-day plant) Yes Fowler, M.R. et al. (2000) J. Exp. Bot. 353, 2125–6.
    PubMed ID #11141186.
    Chicory taproot, etiolated heads, leaf, and green and etiolated seedlings Yes Fowler, M.R. et al. (2000) J. Exp. Bot. 353, 2125–6.
    PubMed ID #11141186.
    Three-day-old mung bean hypocotyls Yes Leiter, H. et al. (1999) J. Biol. Chem. 274, 21830–9.
    PubMed ID #10419500.
    Pea seedling leaf Yes Skaf, J.S. et al. (2000) J. Gen. Virol. 81, 1103–9.
    PubMed ID #10725438.
    Cultured Cells
    Sample Type Promega External Comments
    Cultured cells (Established cell lines are not listed.) Yes A general protocol for total RNA isolation from mammalian cultured cells is provided in the SV Total RNA Isolation System Technical Manual #TM048. Additional resources are listed below.
    Primary rat endothelial cells Yes Thum, T., Haverich, A. and Borlak, J. (2000) FASEB J. 14, 740–51.
    PubMed ID #10744630.
    Fibroblasts derived from mouse embryonic stem cells Yes Zhang, S., Day, I.N.M. and Ye, S. (2001) Physiol. Genomics 5, 187–92.
    PubMed ID #11328964.
    Primary human coronary artery endothelial cells Yes Zhang, S., Day, I.N.M. and Ye, S. (2001) Physiol. Genomics 5, 187–92.
    PubMed ID #11328964.
    Bacteria
    Sample Type Promega External Comments
    Bacteria A general protocol for total RNA isolation from Gram-positive and Gram-negative bacteria is provided in the SV Total RNA Isolation System Technical Manual #TM048. Additional resources are listed below.
    Escherichia coli Yes Yes Follow the standard protocol for bacteria in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Kobs, G. (1998) Promega Notes 68, 28–31.
    Ohmichi, T., Maki, A. and Kool, E.T. (2002) Proc. Natl. Acad. Sci. USA 99, 54–9.
    PubMed ID #11752404.
    Bacillus subtilis Yes Yes Follow the standard protocol for bacteria in the SV Total RNA Isolation System Technical Manual #TM048.
    Additional resources:
    Kobs, G. (1998) Promega Notes 68, 28–31.
    Colombo, M.L. et al. (2004) Antimicrob. Agents Chemother. 48, 484–90.
    PubMed ID #14742199.
    Vibrio vulnificus Yes Chen, Y.C. et al. (2004) Infect. Immun. 72, 629–36.
    PubMed ID #14742502.
    Salmonella enterica Yes Kelly, A. et al. (2004) Microbiology 150, 2037–53.
    PubMed ID #15256548.
    Klein, J.R., Fahlen, T.F. and Jones, B.D. (2000) Infect. Immun. 68, 3368–76.
    PubMed ID #10816487.
    Streptomyces coelicolor Yes The biomass from pregerminated Streptomycetes coelicolor spores was collected, frozen in liquid nitrogen and crushed by mortar and pestle. The resulting powder was processed to extract RNA using the TRIzol® reagent, and the aqueous phase was treated with DNase I. SV RNA Dilution Buffer (375µl) was added to the nucleic acid solution, and samples were mixed and centrifuged. The resulting supernatant wa...
  • Protocols

    Complete Protocol

    Download PDF

    SV Total RNA Isolation System Protocol

    PDF (1 MB)

    Quick Protocols

    Download PDF

    SV Total RNA Isolation System Quick Protocol FB025

    PDF (119 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles

    Citations