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Terminal Deoxynucleotidyl Transferase, Recombinant

by Promega
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  • Overview
  • Protocols
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  • Terminal Deoxynucleotidyl Transferase, Recombinant, catalyzes the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA initiator accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co2+ (as CoCl2) in the reaction buffer allows the tailing of 3´ ends with varying degrees of efficiency. TdT is provided with 5X Reaction Buffer: 500mM cacodylate buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT.

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    Applications

    • Tailing reactions to add complementary homopolymer tails to DNA vectors and cDNA.
    • 3´ end-labeling.
    • TUNEL assays.

    Storage Buffer: 50mM potassium phosphate (pH 6.4), 100mM NaCl, 1mM ?-mercaptoethanol, 0.1% Tween® 20 and 50% glycerol.

    Source: Recombinant E. coli strain.

    QC Tests: Activity, endonuclease, DNase, RNase, 3´ end-labeling, apoptotic DNA end-labeling.

    Unit Definition: One unit of activity catalyzes the transfer of 0.5 picomoles of ddATP to oligo(dT)16 per minute at 37°C in 1X Terminal Transferase Buffer. The resulting oligo(dT)17 is measured by HPLC.

  • Protocols

    Complete Protocol

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    Terminal Deoxynucleotidyl Transferase, Recombinant Protocol

    PDF (112 KB)

  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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