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TFIID Consensus Oligonucleotide

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • The electrophoretic mobility shift assay (EMSA, gel shift, gel retardation) is a relatively simple and sensitive method to investigate protein:DNA interactions. These oligonucleotides contain consensus DNA-binding sites for individual sequence-specific transcription factors. The double-stranded oligonucleotides are designed with 5´ OH blunt ends, making them easily labeled to high specific activity with T4 polynucleotide kinase.

    Applications include gel-shift assays and affinity chromatography.
  • Protocols

    Complete Protocol

    Download PDF

    Gel Shift Assay System Technical Bulletin

    PDF (199 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Citations