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Thermolysin

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Active at High Digestion Temperatures

    Thermolysin is a thermostable metalloproteinase. The high digestion temperature stability of this enzyme may be useful as an alternative to denaturants to improve digestion of proteolytically resistant proteins. Thermolysin preferentially cleaves at the N-terminus of the hydrophobic residues leucine, phenylalanine, valine, isoleucine, alanine and methionine. The optimal digestion temperature range is 65–85°C. Thermolysin activity is optimal at pH 5.0–8.5.

    Applications

    • Peptide mapping
    • Protein identification
    • Characterization of post-translational modifications
    Cut sites for Elastase, Pepsin and Thermolysin.
    Cut sites for Elastase, Pepsin and Thermolysin.
  • Protocols

    Complete Protocol

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    Thermolysin Product Information

    PDF (104 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles