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UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA)

by Promega
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  • Protocols
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  • A Simple, Bioluminescent Assay for Glycosyltransferase Activity

    The UDP-Glo™ Glycosyltransferase Assay provides a homogeneous, single-reagent-addition method to rapidly detect UDP formation in glycosyltransferase reactions. After the glycosyltransferase reaction, an equal volume of UDP Detection Reagent is added to convert the UDP product to ATP and generate light in a luciferase reaction. The light generated is correlated to UDP concentration by using an UDP standard curve.

    This assay is intended for use with purified glycosyltransferases that use UDP-sugar as a donor substrate and cannot be used with whole cells or cell extracts. However, glycosyltransferases purified from cell extracts using immunoprecipitation or affinity tag pulldown can be used in the UDP-Glo™ Assay.

    Applications of the UDP-Glo Assay include profilng glycosyltransferase specificity for different sugars, screening library compounds for effects on glycosyltransferase activity, and detecting chemical compound glucuranidation during drug discovery.

    UDP-Glo™ Glycosyltransferase Assay Reaction: Following the glycosyltransferase reaction, free UDP is converted into ATP, which is then converted into a luminescent signal that is proportional to the glycosyltransferase activity in the reaction.
  • Protocols

    Complete Protocol

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    UDP-Glo™ Glycosyltransferase Assay Technical Manual

    PDF (1 MB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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