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ViviRen Live Cell Substrate

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Generate Kinetic Profiles for Reporter Assays, BRET and RNAi in Living Cells

    ViviRen™ Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence than wildtype coelenterazine. Using live cells, achieve up to 100-fold higher signal-to-noise ratios for super-sensitive quantitation of reporter gene, BRET and RNAi activity.

    Cat.# E6491 is supplied as a liquid, 60mM in DMSO. Cat.# E6492 and E6495 are supplied as a lyophilized solid.

    Assay Advantages

    • Quantitate with confidence using miniaturized formats, low-level expression and CCD imagers.
    • Unparalleled sensitivity with up to 100-fold higher signal-to-noise ratios than coelenterazine.
    • Multiplex with CellTiter-Glo® and other lytic assays for improved accuracy and precision.
    Luminescence reaches near-maximum levels within minutes of ViviRen™ Live Cell Substrate or coelenterazine addition.
    Luminescence reaches near-maximum levels within minutes of ViviRen™ Live Cell Substrate or coelenterazine addition.
  • Protocols

    Complete Protocol

    Download PDF

    ViviRen™ Live Cell Substrate Technical Manual

    PDF (699 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles

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