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VspI

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    AT?TA   AT
    TA   AT?TA

    Usage Note: Ends generated by VspI can be directly ligated to ends generated by NdeI. Neither site is regenerated in the ligation product.

    Incubation Conditions: Buffer D. 37°C.

    Source: Vibrio species.

    Storage Buffer: 20mM Tris-HCl (pH 7.5), 50mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    <10% 25–50% 75–100% 100% <10%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    17 3 2 3 6 1
  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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