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Western Blue Stabilized Substrate for Alkaline Phosphatase

by Promega
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  • Western Blue® Stabilized Substrate for Alkaline Phosphatase is a stable, ready-to-use substrate for Western blots and immunoscreening (e.g., dot blots). It is a mixture of 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitro blue tetrazolium (NBT) in a proprietary stabilizing buffer. Western Blue® Substrate should be used directly and without dilution. This liquid substrate deposits a permanent dark purple stain on membrane sites bearing alkaline phosphatase. Western Blue® Substrate is as sensitive as other reagents based on the BCIP/NBT formulation.


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    Western blot (immunoblot) for ?-actin in cytoplasmic lysate from HEK293T cells.
    Western blot (immunoblot) for ?-actin in cytoplasmic lysate from HEK293T cells.

    Blot performed on a serial dilution of lysate; each lane contains an indicated amount of ?-actin (ng). Primary antibody (monoclonal) used at 1:5,000; Anti-Mouse IgG, AP Conjugate secondary antibody (Cat.# S3721) used at 1:2,500; blot imaged with Western® Blue Stabilized Substrate for Alkaline Phosphatase (Cat.# S3841).

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    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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