ADP-Glo Kinase Assay + PKC mu Kinase Enzyme System

Description

• Overview
• Protocols
• Specifications
• Resources

• 

  Convenient, Scalable Kinase Profiling

  The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PKCu Kinase Enzyme System contains:

  • PKCu Kinase, 10ug (Human, recombinant full-length). MW: ~131kDa.
  • CREBtide (KRREILSRRPSYR) Substrate; derived from human CREB1 isoform A (amino acids 109–121).
  • Reaction Buffer, DTT.

  Recombinant full-length human PKCu was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein Kinase C mu is a member of the protein kinase C (PKC) family that differs from the other PKC isoenzymes in structural and enzymatic properties. PKCu is ubiquitous in nature with the highest expression in the thymus, lung and peripheral blood mononuclear cells. PKCu forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKCu between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases.

  PKCu NCBI Database Entry.

  The PKCu Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

  Use with ADP-Glo™ Kinase Assay

  The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

   

  See all Kinase Enzyme Systems available from Promega.

• Protocols

  Complete Protocol

  

  PKC mu Kinase Enzyme Protocol

  PDF (914 KB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  LESS THAN -65C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Posters

  • Bringing Assays and Automation Together for Lower Throughput Kinase Profiling Poster
  • Automation of ADP-Glo Universal Kinase Assay For HTS Screening AND Profiling Applications Poster
  

  Other

  • Kinome
  • Cell Signaling (An overview)