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Wizard Plus Megapreps DNA Purification System

by Promega
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  • Overview
  • Protocols
  • Specifications
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  • The Wizard® Plus Megapreps DNA Purification System provides a rapid method for large-scale purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. Plasmid DNA yields more than one milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) or TE buffer and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

    Wizard® Plus Megapreps requires the use of a vacuum source and either a lab-assembled or commercial manifold, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) for up to 20 samples or the Vac-Man® Jr. Manifold (Cat.# A7660) for 1 or 2 samples.

  • Protocols

    Complete Protocol

    Download PDF

    Wizard® Plus Megapreps DNA Purification System Technical Bulletin

    PDF (141 KB)

    Quick Protocols

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    Wizard Plus Megapreps DNA Purification System Quick Protocol FB020

    PDF (62 KB)

    Video Protocols

  • Certificate of Analysis

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    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources