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Wizard Plus SV Minipreps DNA Purification System

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® Plus SV Minipreps Start-Up Kits are available. Purchase a Wizard® Plus SV Minipreps DNA Purification System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.

    The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector and can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

  • Protocols

    Complete Protocol

    Download PDF

    Wizard® Plus SV Minipreps DNA Purification System Technical Bulletin

    PDF (975 KB)

    Quick Protocols

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    Wizard Plus SV Minipreps DNA Purification System Quick Protocol FB004

    PDF (72 KB)

    Video Protocols

  • Certificate of Analysis

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    Storage Conditions

    +15C TO +30C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources