- Overview
- Protocols
- Specifications
- Resources
Manual or Automated DNA Extraction in 96-Well Plate Format
The Wizard® SV 96 Genomic DNA Purification System provides a high-throughput, membrane-based technique for consistent preparation of genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step.
With the Wizard® SV Genomic DNA purification system, genomic DNA is purified from cell lysates using 96-well vacuum filtration. Washing the bound DNA requires no disassembly of the manifold, and filtrate waste products are delivered directly to a vacuum trap, eliminating the need to empty waste collection trays.
The Wizard® SV Genomic DNA Purification System is designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments. Validated automated methods available at: www.promega.com/automethods/
For manual purification, the Wizard® SV Genomic DNA Purification System requires a vacuum pump capable of generating 15–20 inches of Hg or the equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of a vacuum trap (not provided).
Learn more about our custom options for this product at: www.promega.com/custom/
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Protocols
Complete Protocol
Wizard® SV 96 Genomic DNA Purification System Technical Bulletin
PDF (463 KB)
Quick Protocols
Wizard SV 96 Genomic DNA Purification System Quick Protocol FB070
PDF (107 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
+15C TO +30C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
Citations
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Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer.
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2016 Nat. Commun.
Software and Firmware
Other
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Acquired RAS or EGFR mutations and duration of response to EGFR blockade in colorectal cancer.