ADP-Glo Kinase Assay + RSK2 Kinase Enzyme System

by Promega
Available SKUs: V9651 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Kinase-background-v2light

    Convenient, Scalable Kinase Profiling

    The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The RSK2 Kinase Enzyme System contains:

    • RSK2 Kinase, 10ug (Human, recombinant full-length). MW: ~112kDa.
    • RSK Substrate (KRRRLSSLRA); derived from human 40S ribosomal protein S6 (amino acid 230–239).
    • Reaction Buffer, DTT.

    Recombinant full-length human RSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK2 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine-threonine kinases. RSK2 has been shown to mediate growth factor signaling via RAS and MAPK, leading to the induction of CREB serine-133 phosphorylation and activation of gene expression. Mutations in RSK2 have been shown to be responsible for Coffin-Lowry syndrome (CLS), which is an X-linked disorder characterized by severe psychomotor retardation, facial and digital dysmorphisms and progressive skeletal deformations.

    RSK2 NCBI Database Entry.

    The RSK2 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

    Use with ADP-Glo™ Kinase Assay

    The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

     

    See all Kinase Enzyme Systems available from Promega.

  • Protocols

    Complete Protocol

    Download PDF

    RSK2 Kinase Enzyme System Protocol

    PDF (1 MB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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