ADP-Glo Kinase Assay + TAK1-TAB1 Kinase Enzyme System

by Promega
Available SKUs: V4089 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Kinase-background-v2light

    Convenient, Scalable Kinase Profiling

    The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The TAK1-TAB1 Kinase Enzyme System contains:

    • TAK1-TAB1 Kinase, 10ug (Human, recombinant; TAK1 (1–303) and TAB1 (437–end)). MW: ~74kDa.
    • Native Swine Myelin Basic Protein (MBP) Substrate.
    • Reaction Buffer, DTT.

    Recombinant human proteins TAK1 (1–303) and TAB1 (437–end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TAK1 is a serine/threonine protein kinase that mediates signaling by TGF? and morphogenetic protein (BMP). In response to IL-1, TAK1 forms a kinase complex with TAB1, and this complex is required for the activation of nuclear factor kappa B (NF-?B). TAK1 also can activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. TAK1 is essential for thymocyte development and activation, and deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 or CD8. Thymocytes lacking TAK1 fail to activate NF-?B and JNK and are prone to apoptosis upon stimulation.

    TAK1 NCBI Database Entry.
    TAB1 NCBI Database Entry.

    The TAK1-TAB1 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

    Use with ADP-Glo™ Kinase Assay

    The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

     

    See all Kinase Enzyme Systems available from Promega.

  • Protocols

    Complete Protocol

    Download PDF

    TAK-TAB1 Kinase Enzyme Protocol

    PDF (924 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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