ADP-Glo Kinase Assay with PI:3PS

by Promega
Available SKUs: V1781 | V1782 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Kinase-background-v2light

    Ready-to-Use Lipid Kinase Substrates and Detection Reagents

    The ADP-Glo™ Kinase Assay with PI:3PS (Cat.# V1781, V1782) contains PI:3PS Lipid Kinase Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.

    The ADP-Glo™ Kinase Assay with PIP2:3PS (Cat.# V1791, V1792) contains PIP2:3PS Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.

    These products are designed for use in screening assays with purified PI3K Class I lipid kinases. The lipid substrates are supplied as frozen small unilamellar vesicles containing a mixture of phosphatidylinositol (PI) or phosphoinositol-4,5-bisphosphate (PIP2) at a 1:3 ratio with phosphatidylserine (PS) as carrier lipid. The PIP2:3PS substrate is optimized for use with class I PI3Ks. The PI:3PS substrate provides a universal PI lipid kinase substrate.

    The ADP-Glo™ Kinase Assay with PI:3PS and the ADP-Glo™ Kinase Assay with PIP2:3PS  are components of the PI3K-Glo™ Class I Profiling Kit (Cat.# V1690).

  • Protocols

    Complete Protocol

    Download PDF

    ADP-Glo™ Lipid Kinase Assay

    PDF (747 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    COMPONENTS AT DIFFERENT TEMPS

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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