AgeI

by Promega
Available SKUs: R7251 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    A?CCGG  T
    T   GGCC?A

    Usage Note: Ends generated with AgeI can be directly ligated to ends generated using AccIII, NgoMIV or XmaI. The AgeI recognition site is not regenerated in the ligation product.

    Incubation Conditions: Buffer K. 37°C.

    Source: Agrobacterium gelatinovorum.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol, 0.5mg/ml BSA.

    Percent Activity in 4-CORE® Buffer:

    A B C D K MULTI-CORE™
    25–50% 25–50% 25–50% 50–75% 100% 100%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    13 5 0 0 0 0
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other

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