AMV Reverse Transcriptase

by Promega
Available SKUs: M5101 | M5108 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Reverse Transcriptase with Stability at Higher Temperatures

    Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

    Features
    • High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20–25u/ul.
    • 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT.
    • Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37–58°C).
    Applications
    • First- and second-strand synthesis of cDNA.
    • Primer extensions and RNA sequencing.
    • RT-PCR. Up to 10ul of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50ul PCR amplification reaction that uses Taq DNA polymerase. If GoTaq® DNA Polymerase or PCR Master Mix are used, up to 25ul of an RT reaction can be added per 50ul PCR.
    Further Reading
    1. Kacian, D.L. (1977) Meth. Virol. 6, 143.
    2. Mierendorf, R.C. and Pfeffer, D. (1987) Meth. Enzymol. 152, 563–6.
  • Protocols

    Complete Protocol

    Download PDF

    AMV Reverse Transcriptase Protocol

    PDF (111 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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