Anti-ACTIVE Caspase-3 pAb

by Promega
Available SKUs: G7481 |
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Description

  • Overview
  • Protocols
  • Specifications
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  • Anti-ACTIVE® Caspase-3 pAb is intended for use as a marker of apoptosis; it specifically stains apoptotic cells without staining nonapoptotic cells. The product includes sufficient antibody to perform 125 immunocytochemical assays (100ul/assay) at a 1:250 dilution.

    Immunogen: Peptide derived from the p17 fragment of caspase-3 and has sequence homology in human, mouse, rat and hamster.

    Antibody form: Affinity-purified rabbit IgG; supplied in Dulbecco's PBS.

    Specificity: Specifically recognizes the cleaved active form of caspase-3 in human, rat and mouse.

    Suggested working dilutions:

    • Immunocytochemistry (1:250)
    • Immunohistochemistry (1:125)
    • Western blotting not recommended

     

    Figure on the right:

    Panel A. Three Anti-ACTIVE® Caspase-3 pAb-positive cells (colored arrows). Panel B. Corresponding DAPI-stained nuclei. Note how the cells labeled with Anti-ACTIVE® Caspase-3 pAb correspond with the typical apoptotic, condensed nuclear morphology in Panel B. Protocols developed and performed at Promega.

     

     

     

     

     

     

     

    Anti-ACTIVE® Caspase-3 pAb
  • Protocols

    Complete Protocol

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    Anti-ACTIVE® Caspase-3 pAb Protocol

    PDF (112 KB)

    Video Protocols

    Apop01-fallback
  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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