4-CORE Buffer Pack (Buffers A, B, C and D), 1ml each

by Promega
Available SKUs: R9921 |
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Description

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  • The 4-CORE® Buffer Pack contains convenient aliquots of Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers.

    See the table of Promega 1X Restriction Enzyme Buffer Composition below.

    1X Restriction Enzyme Buffer Composition.

    Buffer pH (at 37°C) Tris-HCl (mM) MgCl2 (mM) NaCl (mM) KCl (mM) DTT (mM)
    A 7.5 6 6 6 1
    B 7.5 6 6 50 1
    C 7.9 10 10 50 1
    D 7.9 6 6 1,500 1
    E 7.5 6 6 100 1
    F 8.5 10 10 100 1
    G 8.2 50 5
    H 7.5 90 10 50
    J 7.5 10 7 50 1
    K 7.4 10 10 150 1
    L 9.0 10 3 100

    MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.

    Notes:

    1. For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
    2. For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.
    3. All restriction enzymes are supplied with 10mg/ml Acetylated BSA. Although BSA is not absolutely required for activity, it has been shown to enhance activity of many restriction enzymes. We recommend adding BSA to all restriction digests at a final concentration of 0.1mg/ml.
  • Certificate of Analysis

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    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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