ApaI

Description

• Overview
• Protocols
• Specifications
• Resources

• Cut Site:

  G  GGCC^?C
  C_?CCGG  G

  Note: Sensitive to overlapping dcm methylation.

  Incubation Conditions: Buffer A. 37°C.

  Source: Acetobacter pasteurianus.

  Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

  Percent Activity in 4-CORE® Buffer:

  A	B	C	D	MULTI-CORE™
  100%	50–75%	50–75%	<10%	75–100%

  Frequency of Cutting:

  ?	Ad-2	?X174	pUC18	M13mp18	pBR322
  1	12	0	0	0	0

• Protocols

  Complete Protocol

  

  Restriction Enzyme Protocol

  PDF (410 KB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  -30C TO -10C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Other

  • Restriction Enzyme Resource