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Bacterial Strain ES1301 mutS, Glycerol Stock (noncompetent)

by Promega
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  • BMH 71-18 mutS and ES1301 mutS are mismatch repair minus E. colistrains. Use of these strains prevents repair of the newly synthesized unmethylated strand, leading to high mutation efficiencies. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is Tets for cycling between antibiotic resistances. BMH 71-18 is Tetr; therefore, it can only be used to select Ampr or CATr mutants.

    ES1301 mutS Genotype: lacZ53, mutS201::Tn5, thyA36, rha-5, metB1, deoC, IN(rrnD–rrnE).

    BMH 71-18 mutS Genotype: thi, supE, ?(lac-proAB), [mutS::Tn10], [F´, proAB, lacIqZ?M15].


    1. Wallace, R.B. et al. (1981) Gene 16, 21–6.
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    Storage Conditions

    LESS THAN -65C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

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