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BamHI (HC)

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    G?GATC  C
    C   CTAG?G

    Choose Your Configuration: Learn more about our custom options for this product at:

    Usage Notes:

    1. Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/ug, low salt conditions or in the presence of Mn2+.
    2. Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.

    Incubation Conditions: Buffer E. 37°C.

    Source: Bacillus amyloliquefaciens H.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    75–100%* 75–100% 75–100% 50–75% 100% 75–100%

    *Buffer A is not recommended due to potential star activity.

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    5 3 0 1 1 1
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources