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5x-ampsolution-reagent

5X AmpSolution Reagent

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources

  • 5X AmpSolution™ Reagent

    The 5X AmpSolution™ Reagent streamlines the processing of swabs and punches for PowerPlex® STR analysis without compromising your results. While direct amplification from FTA® cards is transforming workflows in databasing and paternity testing labs, buccal swabs and non FTA cards are not ideal substrates for direct amplification.

    Additionally, the AmpSolution™ Reagent enables direct amplification of DNA from unwashed FTA® punches, nonFTA punches and swabs from traditional PowerPlex® kits that were not originally intended for that application, minimizing the need to validate a new system to perform direct amplification.

    Buccal Samples on FTA® Punches

    Two 1.2mm punches from a buccal sample on an FTA® card were amplified using the PowerPlex® ESX 17 System. Prior to amplification, AmpSolution™ Reagent was added to the reaction mix. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).

  • Protocols

    Complete Protocol

    Download PDF

    PunchSolution™ Kit Technical Manual

    PDF (365 KB)

     Download PDF

    SwabSolution™ Kit Technical Manual

    PDF (485 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO +10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Articles