BclI

Description

• Overview
• Protocols
• Specifications
• Resources

• Cut Site:

  T^?GATC  A
  A  CTAG_?T

  Notes:

  1. Sensitive to dam methylation.
  2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  3. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.
  4. Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.

  Incubation Conditions: Buffer C. 50°C. (BclI exhibits 10–25% activity at 37°C.)

  Source: Bacillus caldolyticus.

  Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

  Percent Activity in 4-CORE® Buffer:

  A	B	C	D	MULTI-CORE™
  10–25%	75–100%	100%	50–75%	10–25%

  Frequency of Cutting:

  ?	Ad-2	?X174	pUC18	M13mp18	pBR322
  8	5	0	0	0	0

• Protocols

  Complete Protocol

  

  Restriction Enzyme Protocol

  PDF (410 KB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  -30C TO -10C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Other

  • Restriction Enzyme Resource