BclI

by Promega
Available SKUs: R6651 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    T?GATC  A
    A  CTAG?T

    Notes:

    1. Sensitive to dam methylation.
    2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
    3. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.
    4. Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.

    Incubation Conditions: Buffer C. 50°C. (BclI exhibits 10–25% activity at 37°C.)

    Source: Bacillus caldolyticus.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    10–25% 75–100% 100% 50–75% 10–25%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    8 5 0 0 0 0
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other

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