- Overview
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Cut Site:
T?GATC A
A CTAG?TNotes:
- Sensitive to dam methylation.
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.
- Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.
Incubation Conditions: Buffer C. 50°C. (BclI exhibits 10–25% activity at 37°C.)
Source: Bacillus caldolyticus.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™ 10–25% 75–100% 100% 50–75% 10–25% Frequency of Cutting:
? Ad-2 ?X174 pUC18 M13mp18 pBR322 8 5 0 0 0 0 -
Protocols
Complete Protocol
Restriction Enzyme Protocol
PDF (410 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Other