BDNF Emax ImmunoAssay System

Description

• Overview
• Protocols
• Specifications
• Resources

• The BDNF E_max® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of brain-derived neurotrophic factor (BDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, BDNF in tissue culture supernatants, tissue homogenates, plasma and urine can be quantitated in the range of 7.8–500pg/ml. Binding and recovery from mouse brain homogenates has not been fully characterized.

  Note: Plates are not included.

• Protocols

  Complete Protocol

  

  BDNF E_max® ImmunoAssay System Technical Bulletin

  PDF (252 KB)

• Certificate of Analysis

  Lookup Certificate of Analysis

  Storage Conditions

  -30C TO -10C

  Use Restrictions

  For Research Use Only. Not for Use in Diagnostic Procedures.

• Resources

  

  Articles

  • ELISA In Situ: Sensitive Detection of Endogenous BDNF Release
  • Neuroprotection: Understanding the Modus Operandum of Glutamate Excitotoxicity
  

  Citations

  • Mechanism mediating the enhanced transcription of P2X₃ receptor gene by calcitonin gene related peptide in trigeminal sensory neurons.
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    2008 J. Biol. Chem.