- Overview
- Protocols
- Specifications
- Resources
The BDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of brain-derived neurotrophic factor (BDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, BDNF in tissue culture supernatants, tissue homogenates, plasma and urine can be quantitated in the range of 7.8–500pg/ml. Binding and recovery from mouse brain homogenates has not been fully characterized.
Note: Plates are not included.
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Protocols
Complete Protocol
BDNF Emax® ImmunoAssay System Technical Bulletin
PDF (252 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
- ELISA In Situ: Sensitive Detection of Endogenous BDNF Release
- Neuroprotection: Understanding the Modus Operandum of Glutamate Excitotoxicity
Citations