Beta-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
Simple Detection of ?-Gal Activity in Bacterial and Mammalian Cell Lysates
The ?-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying ?-galactosidase activity in lysates prepared from cells transfected with ?-galactosidase reporter vectors such as the pSV-?-Galactosidase Control Vector.
The standard assay is performed by adding a dilute sample to an equal volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenyl-?-d-galactopyranoside). Samples are incubated for at least 30 minutes, during which time the ?-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow. The reaction can be terminated by addition of sodium carbonate, and the absorbance at 420nm is measured by spectrophotometry.
Cat.# E2000 contains sufficient reagents for 65 standard assays or 200 assays in a 96-well plate format.References
- Miller, J.H., ed. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
- Sambrook, J. et al. (1989) Molecular Cloning, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
- Schenborn, E. and Goiffon, V. (1993) Promega Notes 41, 11–3.
?-Galactosidase Enzyme Assay System with Reporter Lysis Buffer Technical Bulletin
PDF (209 KB)
ß-Galactosidase Enzyme Assay System with Reporter Lysis Buffer Quick Protocol FB051
PDF (73 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.