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BglI

by Promega
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  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    GCCN   NNN?NGGC
    CGGN?NNN   NCCG

    Notes:

    1. BglI recognizes the defined internal six bases of the SfiI recognition site and also cuts at the same position.
    2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.

    Incubation Conditions: Buffer D. 37°C.

    Source: Bacillus globigii.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    10–25% 25–50% 75–100% 100% 100%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    29 20 0 2 1 3
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other