Bright-Glo Luciferase Assay System
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Improved Precision and Reproducibility for High-Throughput Reporter Assays
High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. Bright-Glo™ Luciferase Assay System is designed specifically to meet the needs of continuous-process systems by providing robust, homogeneous assay chemistry that achieves high assay sensitivity and approximately 30-minute signal half-life without prior sample processing. The assay is also less sensitive to mixing conditions, sample evaporation and pipetting errors. These attributes also benefit those who are using fewer samples but still require high sensitivity and ease of use.
- No sample preprocessing. With the Bright-Glo Assay, there is no need to remove culture medium or wash cells prior to adding assay reagent. Grow and assay cells directly in the same multiwell plate.
- High Sensitivity. Up to tenfold more sensitive than other homogenous luciferase assay reagents.
- Convenient and Easy to Use. Simply mix buffer with lyophilized substrate and add to cells in culture medium; no need to thaw or measure before use.
Learn more about custom options for this product at: www.promega.com/custom/
Contact Promega for information on bulk purchases.
Bright-Glo™ Luciferase Assay System Technical Manual
PDF (383 KB)
Bright-Glo and Steady-Glo Luciferase Assay Systems Quick Protocols FB037
PDF (103 KB)
Certificate of AnalysisLookup Certificate of Analysis
-30C TO -10C
For Research Use Only. Not for Use in Diagnostic Procedures.