BstEII

by Promega
Available SKUs: R6641 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Cut Site:

    G?GTNAC   C
    C   CANTG?G

    Notes:

    1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
    2. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.

    Incubation Conditions: Buffer D. 60°C. (BstEII exhibits 25–50% activity at 37°C.)

    Source: Bacillus stearothermophilus.

    Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

    Percent Activity in 4-CORE® Buffer:

    A B C D MULTI-CORE™
    25–50% 50–75% 50–75% 100% 100%

    Frequency of Cutting:

    ? Ad-2 ?X174 pUC18 M13mp18 pBR322
    13 10 0 0 0 0
  • Protocols

    Complete Protocol

    Download PDF

    Restriction Enzyme Protocol

    PDF (410 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

    Other

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