BstEII

Cut Site:

G^?GTNAC   C
C   CANTG_?G

Notes:

1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
2. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.

Incubation Conditions: Buffer D. 60°C. (BstEII exhibits 25–50% activity at 37°C.)

Source: Bacillus stearothermophilus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

Frequency of Cutting: