- Overview
- Protocols
- Specifications
- Resources
Cut Site:
G?GTNAC C
C CANTG?GNotes:
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/ug.
Incubation Conditions: Buffer D. 60°C. (BstEII exhibits 25–50% activity at 37°C.)
Source: Bacillus stearothermophilus.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
A B C D MULTI-CORE™ 25–50% 50–75% 50–75% 100% 100% Frequency of Cutting:
? Ad-2 ?X174 pUC18 M13mp18 pBR322 13 10 0 0 0 0 -
Protocols
Complete Protocol
Restriction Enzyme Protocol
PDF (410 KB)
-
Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
-
Resources
Other