- Overview
- Protocols
- Specifications
- Resources
Simple Protocol Offers Fast, Sensitive Results
The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (u) and 2 (m) activities. Calpains are a family of calcium-activated cysteine proteases involved in cleaving a wide variety of proteins. Calpains modulate the biological activities of their substrates via limited proteolysis.
The Calpain-Glo™ Protease Assay provides a succinyl, proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimized for calpain and luciferase activities. The addition of the calpain reagent in an "add-mix-measure" format results in calpain cleavage of the substrate and rapid development of a "glow-type" luminescent signal produced by the luciferase reaction. The signal is proportional to the amount of calpain activity present. The assay is designed for use with purified enzyme preparations.
- Designed for measuring calpain activity and/or inhibition in a multiwell format and easy to automate.
- Well suited for rapidly autolysed enzymes like calpain; maximum sensitivity is reached in as little as 10 minutes, while the enzyme is fully active.
- Up to 1,000 times more sensitive than competitive fluorometric assays. The luminescent assay avoids inherent fluorescent background signals, providing excellent signal-to-background readings. The assay is linear over 4 logs of calpain concentration.
- Moldoveanu, T. et al. (2002) Cell 108, 649–60.
Sensitivity of the Calpain-Glo™ Protease Assay compared to fluorescent assays. -
Protocols
Complete Protocol
Calpain-Glo™ Protease Assay Technical Bulletin
PDF (404 KB)
-
Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
-
Resources
Articles
- Screen for Calpain Inhibitors Using a Cell-Based High-Throughput Assay
- A Bioluminescent Assay for Calpain Activity
Citations
-
Bioluminescent assays for ADMET
--
2008 Expert Opin. Drug Metab. Toxicol.